Welcome!
The mission of the UOAC Proteomics Core Facility is to provide investigators at the UOAC access to mass spectrometry and gel based proteomics for identification and quantitation of proteins and their posttranslational modifications (PTM).
We have state of-the-art equipment, including two Orbitrap Velos instruments and 5800 MALDI TOF/TOF.
Our workflows for relative protein quantitation are based on DIGE, label-free, iTRAQ/TMT and SILAC approaches. We can also help investigators to identify and quantify protein posttranslational modifications, including phosphorylation, nitrosylation, acetylation, ubiquitination, nitration, etc. We provide training in proper sample preparation and lead the researchers through mass spectrometric analysis to data searching and interpretation. Users have access to a variety of proteomics software platforms (Mascot, Proteome Discoverer, Sequest, Scaffold, Protein Pilot) for re-searching the data or viewing the results. In addition to helping the UOAC investigators, we develop new approaches for PTM characterization and absolute protein quantitation.
Mariano Guzek Ph.D. | Proteomics Core Head
(301) 594-1064
Lab Employees
Mariano Guzek, PhD
Guillermo Lanham, PhD
Yang Chen, PhD
Steve Smirnov, PhD
Guan Wung, PhD
Sarah Pat, MSc
| Hours | Location |
|
Monday - Friday 8:00am - 5:00pm Excluding Holidays |
Heart, Lung, and Blood Institute 10 Center Drive MSC 1774
Office area: Building 10 Room 8C103 Lab area: 8C214, 6N116 (Orbitrap XL) and 5D08 (2D gel and DIGE) |
| ► Service UMG (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Phosphorylation analysis of individual proteins |
TiO2 enrichment, protein and PTM ID, manual validation |
Inquire | |
